一群由穷凶极恶的死刑犯组成的特种小队,小丑女、死亡射手、回旋镖队长、黑蜘蛛、冰霜杀手、鲨鱼王、克格勃野兽等人,受制于植入脑后的纳米炸药,他们被迫受命闯入阿卡姆疯人院,去取得一份被谜语人窃走的秘密名单。小队成员互相猜忌,各怀心思,哥谭的黑暗骑士又会不会任由自杀小队完成任务呢?囚禁于阿卡姆的小丑和其他恶人又会对任务造成怎样的影响?In Gotham City, the Riddler has secret information on the top secret Task Force X that he threatens to make public, but before Amanda Waller can have him killed, he is captured by Batman. Now with Riddler imprisoned in Arkham Asylum, Amanda Waller reassembles the Suicide Squad of captured supervillains with the mission to infiltrate the forensic mental hospital to retrieve Riddler's information. Led by Deadshot, this black ops team makes their way into the facility, only to face complications from within and without the team with secret agendas and double crosses all around. Meanwhile, Batman has his own concerns with an urgent search for The Joker's deadly bomb that threatens Gotham City. Eventually, all these players converge on Arkham Asylum for a showdown that few are going to escape alive.